Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Golgi apparatus 

Staining summary

Mainly localized to the nucleus but also to the cytoplasm and Golgi apparatus.

Reliability (APE)

Low

Antibodies in assay

HPA012050, HPA016959
 

Antibody HPA012050

 

Antibody HPA016959

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Moderate Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular, Aggregate Granular 

Staining intensity

Moderate Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm & golgi apparatus.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus, the Golgi apparatus and cytoplasm in all three cell lines.   Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-251 MG and U-2 OS.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus
Nucleus but not nucleoli