Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleoli, Focal Adhesions

Additional location(s)

Centrosome 

Staining summary

Staining of nucleus and focal adhesions in all three cell lines with additional staining of centrosome in U-251 MG and A-431.

Reliability (Single)

Antibodies in assay

HPA004940
 

Antibody HPA004940

 

         Cell line A-431

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Location(s)

Nucleus, Nucleoli, Centrosome, Focal Adhesions 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli, Focal Adhesions 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli, Centrosome, Focal Adhesions 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus, nucleoli, centrosome & focal adhesions.
 

Summary

Staining of nucleus and focal adhesions in all three cell lines with additional staining of centrosome in U-251 MG and A-431.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Centrosome
Focal adhesions
Nucleoli
Nucleus