Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to the cytoplasm. In addition localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA010691, CAB019287
 

Antibody HPA010691

 

Antibody CAB019287

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  Speckle 

Staining intensity

  Moderate 

Validation

  
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  Speckle 

Staining intensity

  Moderate 

Validation

  
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Speckle 

Staining intensity

Negative Weak 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli