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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Plasma membrane, Cell Junctions

Additional location(s)

 

Staining summary

Localized to the nucleus, plasma membrane and cell junctions.

Reliability (APE)

Medium

Antibodies in assay

HPA012132, HPA016873
 

Antibody HPA012132

 

Antibody HPA016873

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Plasma membrane, Cytoplasm, Cell Junctions 

Staining characteristics

Granular  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Plasma membrane, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm, Cell Junctions 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, cytoplasm & cell junctions.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines.   Staining of cytoplasm and plasma membrane/cell junctions in all three cell lines with additional staining of nucleus in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cell junctions
Nucleus but not nucleoli
Plasma membrane