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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Vesicles, Centrosome 

Staining summary

Localized to plasma membrane but also centrosome and vesicles.

Reliability (APE)

High

Antibodies in assay

HPA009672, HPA027247
 

Antibody HPA009672

 

Antibody HPA027247

 

         Cell line A-431

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Location(s)

Plasma membrane, Vesicles Plasma membrane, Centrosome 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Plasma membrane, Vesicles Plasma membrane, Centrosome 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane, Vesicles Plasma membrane, Centrosome 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & vesicles.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & centrosome.
 

Summary

Staining of vesicles and plasma membrane in all three cell lines.   Staining of the plasma membrane and the centrosome in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Plasma membrane
Endosome