Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleoli

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus and nucleoli. In addition localized to the cytoplasm.

Reliability (APE)

Low

Antibodies in assay

HPA003282, CAB020828
 

Antibody HPA003282

 

Antibody CAB020828

 

         Cell line A-431

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Location(s)

Nucleus, Nucleoli, Cytoplasm  

Staining characteristics

Cluster  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleoli, Cytoplasm  

Staining characteristics

Granular, Cluster  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli, Cytoplasm  

Staining characteristics

Spotty  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus, nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Summary

Staining of cytoplasm, nucleus and nucleoli in all three cell lines.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus