Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Nucleoli, Nuclear membrane 

Staining summary

Localized to the nucleus, including nucleoli and the nuclear membrane.

Reliability (APE)

Medium

Antibodies in assay

HPA015315, HPA015801
 

Antibody HPA015315

 

Antibody HPA015801

 

         Cell line A-431

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Location(s)

Nucleus, Nucleoli, Nuclear membrane Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli Nucleus 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli, Nuclear membrane Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus, nucleoli & nuclear membrane.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus and nucleoli in all three cell lines with additional staining of nuclear membrane in U-251 MG and A-431.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nuclear membrane
Nucleoli
Nucleus