Fields »

SUBCELLULAR LOCATION

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Main location(s)

Vesicles

Additional location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining summary

Mainly localized to vesicles.

Reliability (APE)

Low

Antibodies in assay

HPA038052, HPA038053
 

Antibody HPA038052

 

Antibody HPA038053

 

         Cell line A-431

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Location(s)

Vesicles Nucleus but not nucleoli 

Staining characteristics

Granular  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Vesicles Vesicles 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Vesicles Nucleus but not nucleoli, Vesicles 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & vesicles.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & vesicles.
 

Summary

Staining of vesicles in all three cell lines with additional staining of cytoplasm in U-251 MG.   Staining of nucleus in A-431. Staining of vesicles in U-2 OS. Staining of both nucleus and vesicles in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Endosome