Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleoli

Additional location(s)

Nucleus 

Staining summary

Mainly localized to nucleoli. In addition localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA041117, HPA041540
 

Antibody HPA041117

 

Antibody HPA041540

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus, Nucleoli 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli Nucleus, Nucleoli 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli Nucleus, Nucleoli 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & nucleoli.
 

Summary

Staining of nucleus and nucleoli in all three cell lines.   Staining of nucleus and nucleoli in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleoli
Nucleus