Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nuclear membrane

Additional location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining summary

Staining of nucleus, nuclear membrane and cytoplasm in all three cell lines.

Reliability (Single)

Antibodies in assay

HPA011785
 

Antibody HPA011785

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Nuclear membrane, Cytoplasm 

Staining characteristics

Fibrous 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Nuclear membrane, Cytoplasm 

Staining characteristics

Smooth 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Nuclear membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, nuclear membrane & cytoplasm.
 

Summary

Staining of nucleus, nuclear membrane and cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nuclear membrane
Nucleus but not nucleoli