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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and the nucleus.

Reliability (APE)

Low

Antibodies in assay

HPA008399, CAB015221, HPA017017
 

Antibody HPA008399

 

Antibody CAB015221

 

Antibody HPA017017

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular   

Staining intensity

Moderate Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Nucleus but not nucleoli   

Staining characteristics

    

Staining intensity

Negative Weak   

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
   

Comment

    

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Negative Moderate Strong 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in A-431.   Staining of nucleus in all three cell lines.   Staining of nucleus and cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli