The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human breast shows strong nuclear and extracellular positivity in glandular cells.
Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Summary
Most of the normal tissues displayed moderate to strong nuclear and cytoplasmic positivity. Hepatocytes and bile duct cells were weakly stained or negative.
Strong nuclear positivity was observed in breast. Exocrine pancreas, adrenal cortex, Stomach, renal tubules, neuronal cells and cardiac myocytes showed moderate cytoplasmic staining. A subset of cells in intestinal tract and squamous epithelia showed moderate nuclear staining. Remaining normal tissues were generally negative.
Normal tissues showed weak to moderate cytoplasmic and nuclear staining. Purkinje cells, cells in seminiferus ducts and neuronal cells of lateral ventricles exhibited strong cytoplasmic positivity while adrenal displayed additional strong nuclear immunoreactivity. Additional membranous positivity was observed in a fraction of tissues. Liver, parathyroid and the glial cells along with lymphoid tissues were negative or weakly stained.
Most of the normal tissues displayed moderate cytoplasmic positivity with additional nuclear staining in many tissues. Strong staining was observed in muscle and Purkinje cells.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
