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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Vesicles 

Staining summary

Mainly localized to the nucleus. In addition localized to the cytoplasm or vesicles.

Reliability (APE)

Low

Antibodies in assay

CAB017832, HPA029974, HPA029975, HPA029976
 

Antibody CAB017832

 

Antibody HPA029974

 

Antibody HPA029975

 

Antibody HPA029976

 

         Cell line A-431

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Location(s)

Nucleus, Nucleoli Nucleus but not nucleoli, Cytoplasm  Nucleus but not nucleoli, Vesicles 

Staining characteristics

    

Staining intensity

Strong Weak Negative Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm  Nucleus but not nucleoli, Vesicles 

Staining characteristics

    

Staining intensity

Strong Weak Negative Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli, Cytoplasm Nucleus but not nucleoli  Nucleus but not nucleoli, Vesicles 

Staining characteristics

    

Staining intensity

Moderate Strong Negative Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & vesicles.
 

Summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-251 MG and U-2 OS.   Staining of nucleus in all three cell lines with additional staining of cytoplasm in A-431 and U-2 OS.   No staining was observed in any of the three cell lines.   Staining of nucleus and vesicles in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Endosome