The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human bronchus shows strong cytoplasmic and membranous positivity in respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows strong cytoplasmic and membranous positivity in respiratory epithelial cells.
Immunohistochemical staining of human fallopian tube shows strong positivity in ciliated cells.
Summary
Strong cytoplasmic and membranous immunoreactivity was observed in gall bladder and seminal vesicle as well as ciliated cells in respiratory epithelia and fallopian tube while intestinal paneth cells showed strong cytoplasmic positivity. Gastric mucosa and prostate were moderately stained. Remaining normal tissues were generally negative.
Ciliated cells showed strong cytoplasmic and membranous staining. Luminal membranous staining was observed in renal tubuels and small intestine. The remaining normal tissues were weakly stained or negative.
Most normal tissues showed weak to moderate, often granular, cytoplasmic positivity. Ciliated cells displayed strong cytoplasmic and membranous staining while gastric mucosa, exocrine pancreas, renal tubules, endometrial glands and prostate were showed strong cytoplasmic positivity. Liver, glial cells and most lymphoid cells were negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
