Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA031196, HPA031197, HPA031198
 

Antibody HPA031196

 

Antibody HPA031197

 

Antibody HPA031198

 

         Cell line A-431

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Location(s)

Nucleus Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Moderate Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus   Nucleus but not nucleoli 

Staining characteristics

    

Staining intensity

Weak   Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
   
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Moderate Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in all three cell lines. Additional staining of nucleoli in U-251 MG.   Staining of nucleus in U-251 MG and A-431.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus