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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)

Additional location(s)

 

Staining summary

Localized to the nucleus and microtubules.

Reliability (APE)

Very low

Antibodies in assay

HPA028774, HPA028775
 

Antibody HPA028774

 

Antibody HPA028775

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)   

Staining characteristics

   

Staining intensity

Moderate   

Validation

Location not consistent with experimental gene/protein characterization data.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)  

Staining characteristics

Speckle  

Staining intensity

Strong Negative 

Validation

Location not consistent with experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)  

Staining characteristics

Speckle  

Staining intensity

Moderate Negative 

Validation

Location not consistent with experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoskeleton (microtubules).
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Summary

Staining of nucleus and cytoskeleton (microtubules) in all three cell lines.   No staining observed in U-251 MG and U-2 OS.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Microtubules
Nucleus but not nucleoli