Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Centrosome 

Staining summary

Mainly localized to the plasma membrane and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA029135, HPA029136
 

Antibody HPA029135

 

Antibody HPA029136

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Cell Junctions Plasma membrane, Cytoplasm, Centrosome 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm, Centrosome 

Staining characteristics

Granular  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Cytoplasm Plasma membrane, Cytoplasm, Centrosome 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & centrosome.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of plasma membrane in U-2 OS and nucleus, plasma membrane and cell junctions in A-431.   Staining of cytoplasm, plasma membrane and centrosome in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Plasma membrane