Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Plasma membrane, Golgi apparatus 

Staining summary

Mainly localized to the cytoplasm. In addition localized to the Golgi apparatus or the plasma membrane.

Reliability (APE)

Medium

Antibodies in assay

HPA031608, HPA031609
 

Antibody HPA031608

 

Antibody HPA031609

 

         Cell line A-431

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Location(s)

Cytoplasm, Golgi apparatus Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Golgi apparatus Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Golgi apparatus Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in golgi apparatus.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of the Golgi apparatus in all three cell lines and cytoplasm U-251 MG and A-431.   Staining of cytoplasm and plasma membrane in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Plasma membrane