Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus but also to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA012383, HPA012384
 

Antibody HPA012383

 

Antibody HPA012384

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus 

Staining characteristics

 Smooth 

Staining intensity

Weak Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Weak Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines.   Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus