Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Plasma membrane, Cytoplasm 

Staining summary

Mainly localized to the nucleus and cytoplasm. In addition localized to plasma membrane.

Reliability (APE)

High

Antibodies in assay

HPA036913, HPA036914
 

Antibody HPA036913

 

Antibody HPA036914

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus, Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus, Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm   

Staining characteristics

   

Staining intensity

Weak   

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, plasma membrane & cytoplasm.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines.   Staining of nucleus, cytoplasm and plasma membrane in A-431 and U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus
Plasma membrane