MAPKAPK3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA058275

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA058275 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

39 

Antigen sequence

RVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNN
 

Matching transcripts

MAPKAPK3-001 - ENSP00000350639 [100%]
MAPKAPK3-002 - ENSP00000396467 [100%]
MAPKAPK3-007 - ENSP00000394894 [100%]
MAPKAPK3-201 - ENSP00000478922 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line PC-3 shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:88 

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

43.0, 11.0 

Antibody dilution

1:300 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3550 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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MAPKAPK3-001
 
MAPKAPK3-002
 
MAPKAPK3-007
 
MAPKAPK3-201