Fields »

SUBCELLULAR LOCATION

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Main location(s)

Mitochondria

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to mitochondria but also to the cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA010611, HPA011990
 

Antibody HPA010611

 

Antibody HPA011990

 

         Cell line A-431

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Weak Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm, Mitochondria Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Weak Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Mitochondria  

Staining characteristics

Granular  

Staining intensity

Weak Negative 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm & mitochondria.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Summary

Staining of mitochondria and cytoplasm in U-251 MG and U-2 OS. Staining of cytoplasm in A-431.   Staining of cytoplasm in A-431 and U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Mitochondria