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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and nucleus.

Reliability (APE)

High

Antibodies in assay

HPA000931, HPA000982
 

Antibody HPA000931

 

Antibody HPA000982

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleoli in U-251 MG.   Staining of cytoplasm and nucleus but not nucleoli in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli