STK25

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA047147

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA047147 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

9 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

29 

Antigen sequence

PGISDKLMVHLVERVQRFSHNRNHLTSTR
 

Matching transcripts

STK25-001 - ENSP00000325748 [100%]
STK25-002 - ENSP00000384162 [100%]
STK25-003 - ENSP00000385541 [100%]
STK25-004 - ENSP00000384444 [100%]
STK25-005 - ENSP00000385687 [100%]
STK25-201 - ENSP00000446008 [100%]
STK25-202 - ENSP00000444886 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in astrocytes.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:150 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

48.1, 39.3, 37.3 

Antibody dilution

1:270 

Validation WB

Uncertain: No bands detected 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2200 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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STK25-001
 
STK25-002
 
STK25-003
 
STK25-004
 
STK25-005
 
STK25-201
 
STK25-202