Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus but excluded from the nucleoli.

Reliability (APE)

High

Antibodies in assay

CAB003769, HPA022134
 

Antibody CAB003769

 

Antibody HPA022134

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nuclei but not nucleoli.
 

Summary

Staining of nucleus in all three cell lines.   Staining of nuclei but not nucleoli in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli