Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and nucleus.

Reliability (APE)

Very low

Antibodies in assay

HPA027143, HPA028328
 

Antibody HPA027143

 

Antibody HPA028328

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

   

Staining intensity

Strong   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
   

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Summary

Staining of nuclei in all three cell lines.   Staining of cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli