Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Golgi apparatus, Vesicles 

Staining summary

Mainly localized to cytoplasm but also to Golgi apparatus and vesicles.

Reliability (APE)

Medium

Antibodies in assay

HPA021921, HPA023290
 

Antibody HPA021921

 

Antibody HPA023290

 

         Cell line A-431

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Location(s)

Cytoplasm Golgi apparatus, Vesicles 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in golgi apparatus & vesicles.
 

Summary

Staining of cytoplasm in all three cell lines.   Staining of the Golgi apparatus and vesicles in A-431. Staining of the cytoplasm in U-251 MG and U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Golgi apparatus
Endosome