Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Nucleoli 

Staining summary

Localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

CAB002430, HPA003883
 

Antibody CAB002430

 

Antibody HPA003883

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleoli, Nucleus but not nucleoli 

Staining characteristics

Granular Cluster, Spotty, Speckle 

Staining intensity

Weak Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Nucleoli, Nucleus but not nucleoli 

Staining characteristics

 Cluster, Spotty, Speckle 

Staining intensity

Negative Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleoli, Nucleus but not nucleoli 

Staining characteristics

Granular Spotty, Speckle 

Staining intensity

Weak Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & nucleus but not nucleoli.
 

Summary

Staining of nuclei but not nucleoli in U-251 MG and A-431.   Speckled staining of nucleus with spots of nucleoli, in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleoli
Nucleus but not nucleoli