Fields »

SUBCELLULAR LOCATION

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Main location(s)

Mitochondria

Additional location(s)

Nucleus but not nucleoli, Nuclear membrane, Cytoplasm 

Staining summary

Mainly localized to mitochondria. Additionally localized to the cytoplasm and nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA028100, HPA028134
 

Antibody HPA028100

 

Antibody HPA028134

 

         Cell line A-431

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Location(s)

Nuclear membrane, Mitochondria Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nuclear membrane, Mitochondria Cytoplasm, Mitochondria 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nuclear membrane Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclear membrane & mitochondria.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & mitochondria.
 

Summary

Staining of nuclear membrane in all three cell lines with additional staining of mitochondria in A-431 and U-2 OS.   Staining of cytoplasm in all three cell lines with additional staining of nucleus in A-431 and mitochondria in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Nuclear membrane
Nucleus but not nucleoli