Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus

Additional location(s)

 

Staining summary

Localized to cytoplasm, nucleus and the Golgi apparatus.

Reliability (APE)

Medium

Antibodies in assay

CAB013090, HPA026441
 

Antibody CAB013090

 

Antibody HPA026441

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm, Golgi apparatus 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

  Plasma membrane, Cytoplasm 

Staining characteristics

   

Staining intensity

  Strong 

Validation

  
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus but not nucleoli, Golgi apparatus 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm in A-431 and U-251 MG.   Staining of plasma membrane and cytoplasm in U-2 OS. Staining of nucleus and the Golgi apparatus in A-431 and U-251 MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus but not nucleoli