Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleoli

Additional location(s)

Nucleus 

Staining summary

Mainly localized to nucleoli but also to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA026512, HPA028277
 

Antibody HPA026512

 

Antibody HPA028277

 

         Cell line A-431

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Location(s)

Nucleoli Nucleoli 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleoli Nucleoli 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nucleus, Nucleoli 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli.
 

Summary

Staining of nucleoli in A-431 and U-2 OS cells.   Staining of nucleoli in all three cell lines with additional staining of nucleus in U-251 MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleoli
Nucleus