ATP6V0B

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA044281

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA044281 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

26 

Antigen sequence

EPFSATDPKAIGHRNYHAGYSMFGAG
 

Matching transcripts

ATP6V0B-001 - ENSP00000431605 [100%]
ATP6V0B-004 - ENSP00000236067 [100%]
ATP6V0B-006 - ENSP00000432754 [100%]
ATP6V0B-011 - ENSP00000434094 [100%]
ATP6V0B-012 - ENSP00000434729 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human uterus, post-menopause, shows strong cytoplasmic positivity in a subset of ciliated cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:800 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

27.7, 26.1, 21.4, 16.2 

Antibody dilution

1:250 

Validation WB

Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ATP6V0B-001
 
ATP6V0B-004
 
ATP6V0B-006
 
ATP6V0B-011
 
ATP6V0B-012