The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human pancreas shows strong cytoplasmic and nuclear positivity in exocrine cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemical staining of human pancreas shows cytoplasmic positivity both in exocrine glandular cells and islet cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine cells.
Summary
A majority of normal cells showed moderate to strong cytoplasmic immunoreactivity, in several cases was combined with nuclear positivity. Squamous epithelia, lymphoid tissues and cells in CNS were generally negative.
Respiratory epithelia, gastrointestinal tract, hepatocytes, exocrine pancreas and Leydig cells displayed moderate cytoplasmic and nuclear staining whereas renal tubules were strongly stained. Remaining normal tissues were weakly stained or negative.
Most normal tissues showed strong cytoplasmic staining, often with a granular pattern. Renal glomeruli and skeletal muscle were generally negative.
Most normal cells showed moderate nuclear positivity. Exocrine pancreas and renal tubuli displayed strong staining. Liver, spleenic white pulp and myocytes were negative.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
