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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the nucleus and the cytoplasm.

Reliability (APE)

Low

Antibodies in assay

CAB006246, HPA017919, HPA019649, HPA027734
 

Antibody CAB006246

 

Antibody HPA017919

 

Antibody HPA019649

 

Antibody HPA027734

 

         Cell line A-431

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Location(s)

 Cytoplasm Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Granular   

Staining intensity

Negative Moderate Weak Moderate 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-2 OS

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Location(s)

 Cytoplasm Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Granular   

Staining intensity

Negative Moderate Moderate Strong 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

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Location(s)

 Cytoplasm Nucleus but not nucleoli, Cytoplasm Nucleus, Nucleoli 

Staining characteristics

    

Staining intensity

Negative Weak Moderate Strong 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

No staining was observed in any of three cell lines.   Staining of cytoplasm in all three cell lines.   Staining of cytoplasm and nuclei but not nucleoli in all three cell lines.   Staining of nucleus in all three cell lines with additional staining of nucleoli in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus