Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleoli 

Staining summary

Mainly localized to the cytoplasm but also to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA025766, HPA028659
 

Antibody HPA025766

 

Antibody HPA028659

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines.   Staining of nucleoli and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleoli