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SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Nucleoli, Cytoplasm, Golgi apparatus 

Staining summary

Staining of nucleus in all three cell lines with additional staining of Golgi apparatus in U-251 MG and A-431 and cytoplasm in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA034596
 

Antibody HPA034596

 

         Cell line A-431

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Location(s)

Nucleus, Golgi apparatus 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli, Golgi apparatus 

Staining characteristics

 

Staining intensity

Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus & golgi apparatus.
 

Summary

Staining of nucleus in all three cell lines with additional staining of Golgi apparatus in U-251 MG and A-431 and cytoplasm in U-2 OS.  

Validation

The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleoli
Nucleus