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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm, Centrosome

Additional location(s)

 

Staining summary

Localized to the nucleus, centrosome and cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

HPA040764, HPA041091
 

Antibody HPA040764

 

Antibody HPA041091

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Centrosome Cytoplasm 

Staining characteristics

Spotty  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Centrosome  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Centrosome  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & centrosome.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Summary

Staining of centrosome and nucleus in all three cell lines.   Staining of cytoplasm in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli