Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Mainly localized to the nucleus but excluded from nucleoli.

Reliability (APE)

High

Antibodies in assay

HPA005695, CAB022068, CAB035999
 

Antibody HPA005695

 

Antibody CAB022068

 

Antibody CAB035999

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli  Nucleus but not nucleoli 

Staining characteristics

Granular   

Staining intensity

Weak Negative Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli    

Staining characteristics

Granular    

Staining intensity

Moderate Negative   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
   

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular   

Staining intensity

Moderate Negative Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus in all three cell lines.   No staining was observed in any of the three cell lines.   Staining of nucleus in U-251 Mg and A-431 with additional staining of cytoplasm in U-251 MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli