Fields »

SUBCELLULAR LOCATION

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Main location(s)

Mitochondria

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to mitochondria but also to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA029072, HPA029073, HPA029074, HPA029075
 

Antibody HPA029072

 

Antibody HPA029073

 

Antibody HPA029074

 

Antibody HPA029075

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli, Mitochondria    

Staining characteristics

     

Staining intensity

Negative Moderate Negative   

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
No staining.
   

Comment

     

         Cell line U-2 OS

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Location(s)

 Mitochondria  Mitochondria 

Staining characteristics

    

Staining intensity

Negative Moderate Negative Moderate 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

    

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Mitochondria   

Staining characteristics

    

Staining intensity

Negative Weak Negative Negative 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
No staining.
 
No staining.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
 

Summary

No staining was observed in the three cell lines.   Staining of mitochondria in all three cell lines with additional staining of nucleus in U-251 MG and A-431.   No staining was observed in any of the three cell lines.   Staining of mitochondria in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Mitochondria
Nucleus but not nucleoli