Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Cytoplasm

Additional location(s)

Nucleus, Mitochondria 

Staining summary

Mainly localized to cytoplasm but also to nucleus and mitochondria.

Reliability (APE)

Medium

Antibodies in assay

HPA025736, HPA025765
 

Antibody HPA025736

 

Antibody HPA025765

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nucleus, Nucleoli, Cytoplasm Cytoplasm, Mitochondria 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoplasm, Mitochondria 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoplasm, Mitochondria 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & mitochondria.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines.   Staining of cytoplasm and mitochondria in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus