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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoskeleton (Intermediate filaments)

Additional location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining summary

Staining of nucleus, plasma membrane and cytoplasm in U-251MG. Staining of cytoskeleton (intermediate filaments) and plasma membrane in A-431. Staining of cytoskeleton (intermediate filaments) in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA008519
 

Antibody HPA008519

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Cytoskeleton (Intermediate filaments) 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Summary

Staining of nucleus, plasma membrane and cytoplasm in U-251MG. Staining of cytoskeleton (intermediate filaments) and plasma membrane in A-431. Staining of cytoskeleton (intermediate filaments) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments
Nucleus but not nucleoli
Plasma membrane