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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Granular staining of nuclei and cytoplasm in U-251MG and A-431. Granular staining of cytoplasm in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA001755
 

Antibody HPA001755

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Granular staining of nuclei and cytoplasm in U-251MG and A-431. Granular staining of cytoplasm in U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli