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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to nucleus and cytoplasm.

Reliability (APE)

High

Antibodies in assay

CAB002060, HPA028675
 

Antibody CAB002060

 

Antibody HPA028675

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Strong 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus and cytoplasm in A-431 and U-251 MG.   Staining of cytoplasm in all three cell lines with additional staining of nucleus in U-251 MG and A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli