The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Immunohistochemical staining of human tonsil shows strong membranous and cytoplasmic positivity in squamous epithelial cells along with strong positivity in germinal center cells and non-germinal center cells.
Summary
Normal tissues showed moderate to strong cytoplasmic positivity with additional nucleolar staining in many cases. The liver, pancreas, hematopoietic cells, glial cells and the adrenal were weakly stained or negative.
Most of the normal cells displayed weak to moderate cytoplasmic and/or nuclear positivity. Purkinje cells were strongly stained. Spleen, glial cells and placental trophoblasts were negative.
Squamous epithelia, lung, salivary and thyroid gland as well as breast, hepatocytes, renal tubules, lymphoid tissues and endothelial cells displayed strong cytoplasmic and/or membranous positivity. Remaining normal tissues were negative.
Validation
One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data
The multi-targeting antibody yielding a staining pattern partly consistent with limited gene/protein characterization data or there is no available gene/protein characterization data for all or at least one of the genes
The multi-targeting antibody yielding a staining pattern partly consistent with limited gene/protein characterization data or there is no available gene/protein characterization data for all or at least one of the genes
