The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules with distinct extracellular material.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in cells in and outside the reaction centra.
Summary
Most normal tissues showed moderate cytoplasmic staining. Proximal renal tubules were strongly positivity. Additional nuclear staining was for example observed in glial cells and cells in testicular seminiferous ducts.
A majority of normal tissues showed moderate to strong cytoplasmic positivity combined with cases of membranous staining. Spleen, cells in CNS, myocytes, squamous epithelium, testis and bone marrow poetic cells were weakly stained or negative.
A majority of normal tissues showed weak to moderate cytoplasmic positivity. Cells in CNS, myocytes, the female genitalia tract, urinary bladder and most squamous epithelium were generally negative.
Most normal tissues displayed weak to moderate cytoplasmic positivity. Strong staining was observed in lymphoid tissues and bone marrow poietic cells while myocytes were negative.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
