Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleoli, Focal Adhesions 

Staining summary

Mainly localized to the cytoplasm but also detected in focal adhesion sites and nucleoli.

Reliability (APE)

Medium

Antibodies in assay

HPA023094, HPA023660, HPA023779
 

Antibody HPA023094

 

Antibody HPA023660

 

Antibody HPA023779

 

         Cell line A-431

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Location(s)

Cytoplasm  Nucleoli, Cytoplasm, Golgi apparatus 

Staining characteristics

   

Staining intensity

Weak Negative Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm Nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Moderate Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoplasm  Cytoplasm, Focal Adhesions 

Staining characteristics

Granular   

Staining intensity

Weak Negative Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines.   Staining of cytoplasm in U-2 OS.   Staining of cytoplasm in all three cell lines with additional staining of focal adhesions in U-251 MG, nucleoli and Golgi apparatus in A-431 and nucleoli in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Focal adhesions
Nucleoli