The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in leydig cells.
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic and membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Summary
Moderate cytoplasmic staining and occasional nuclear positivity was observed in squamous epithelia, respiratory epithelia, hepatocytes and skeletal myocytes. Esophagus, the adrenal gland and testicular leydig cells were strongly stained. Remaining normal tissues were weakly stained or negative.
Squamous epithelia, respiratory epithelium and urothelium showed strong cytoplasmic and membranous staining. Parts of the intestine showed moderate immunoreactivity while cytotrophoblasts exhibited moderate membranous staining.
Strong cytoplasmic immunoreactivity with a granular pattern was observed in exocrine pancreas, intestinal Paneth cells and adrenal gland. Smooth muscle were distinctly stained. Alveolar macrophages, thyroid gland and neuronal cells were moderately stained. Remaining normal tissues were generally negative.
Validation
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
