Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA007327, HPA008408
 

Antibody HPA007327

 

Antibody HPA008408

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm  

Staining characteristics

Smooth  

Staining intensity

Strong Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines.   Staining of cytoplasm and nucleus in A-431. Staining of cytoplasm in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm