Fields »

SUBCELLULAR LOCATION

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Main location(s)

Endoplasmic reticulum

Additional location(s)

Mitochondria, Vesicles 

Staining summary

Mainly localized to the endoplasmic reticulum. Also detected in the mitochondria and vesicles.

Reliability (APE)

Medium

Antibodies in assay

HPA007599, HPA026593
 

Antibody HPA007599

 

Antibody HPA026593

 

         Cell line A-431

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Location(s)

Mitochondria Vesicles 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Mitochondria, Endoplasmic reticulum Endoplasmic reticulum, Vesicles 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Endoplasmic reticulum Endoplasmic reticulum, Vesicles 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in endoplasmic reticulum.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in endoplasmic reticulum & vesicles.
 

Summary

Staining of endoplasmic reticulum in U-251MG and U-2 OS with additional staining of mitochondria in U-2 OS. Staining of mitochondria in A-431.   Staining of endoplasmic reticulum and vesicles in U-251 MG and U-2 OS. Staining of vesicles in A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Endoplasmic reticulum
Mitochondria
Endosome