Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Centrosome 

Staining summary

Mainly localized to the nucleus but also to the cytoplasm and centrosome.

Reliability (APE)

Low

Antibodies in assay

HPA004799, CAB019284, HPA022271
 

Antibody HPA004799

 

Antibody CAB019284

 

Antibody HPA022271

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli Centrosome 

Staining characteristics

   

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 
No staining.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli   

Staining characteristics

Granular    

Staining intensity

Strong Weak   

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
   

Comment

    

         Cell line U-251 MG

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Location(s)

 Centrosome Nucleus but not nucleoli, Cytoplasm, Centrosome 

Staining characteristics

   

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli, cytoplasm & centrosome.
 

Summary

Staining of cytoplasm and nucleus in U-2 OS.   Staining of nucleus in A-431 and U-2 OS and centrosome in U-251 MG.   Staining of centrosome, cytoplasm and nucleus in U-251 MG. Staining of centrosome in A-431.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is not consistent with literature.
 
The subcellular location is not consistent with literature.
 

Dictionary

Centrosome
Cytoplasm
Nucleus
Nucleus but not nucleoli